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QIAamp 96 DNA QIAcube HT Kit

For automated high-throughput isolation of total DNA from blood, cells, and tissues

  • Simple and reliable automated processing for cost and time savings
  • Standardized method suitable for a variety of sample types
  • High yields even from specialized samples
  • High-quality DNA that performs well in downstream analyses
  • Optimized protocols for a range of starting materials

The QIAamp 96 DNA QIAcube HT Kit enables simple, automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from blood, cells, and tissue samples. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality nucleic acids that are ready for downstream analysis. This kit is designed to be used on QIAcube HT System.

Артикул: 950067
QIAcube HT Plasticware
Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
Артикул: 51331
QIAamp 96 DNA QIAcube HT Kit (5)
For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers


Performance

The QIAamp 96 DNA QIAcube HT Kit provides fast and reliable automated purification of total DNA in 96-well format. Results are highly reproducible, with no significant well-to-well variation in DNA recovery.

Automated purification of total DNA from blood samples is quick, easy, and reliable with the QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed, and the resulting DNA is of the same high quality as from other QIAGEN purification solutions.

QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit lets users increase sample purification throughput without having to compromise on quality or reliability. The procedure provides high yields of pure DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions.

Principle

The QIAamp 96 DNA QIAcube HT Kit enables automated purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen tissues, cells, and blood. Samples treated with common anticoagulants such as citrate, heparin, or EDTA are suitable for use. The procedure yields high-quality DNA that performs well in PCR and other enzymatic reactions.

The QIAamp 96 DNA QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.

Kit specifications

Specification Description
Number of samples 24–96 samples (to be processed in increments of 8)
Sample input volume Blood: up to 200 ?l (for sample volumes less than 200 ?l, add PBS)
Cells: up to 5 x 106 cells
Tissues: up to 20 mg tissue (10 mg for spleen, liver, or lung tissue)
Elution volume 200 ?l
Duration 96 samples in approximately 96 minutes
24 samples in approximately 45 minutes

Procedure

The QIAamp 96 DNA QIAcube HT procedure is fast and simple with the QIAcube HT instrument. Samples are first lysed using QIAGEN Proteinase K. Buffering conditions are adjusted to provide optimal DNA binding conditions and the lysate is loaded onto the QIAamp 96 plate. As vacuum is applied, DNA selectively binds to the QIAamp membrane, and contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in three efficient wash steps. Pure DNA is eluted under vacuum in a single step in 200 µl of Buffer AE.

Applications

The QIAamp 96 DNA QIAcube HT Kit provides high-quality DNA, ready to use in various downstream assays, including applications in:

  • Life science research
  • Livestock breeding
  • Pedigree genotyping
  • Veterinary pathogen research
  • Routine applied testing