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Каталог

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QIAamp DNA Blood BioRobot 9604 Kit

For automated purification of genomic and mitochondrial DNA from whole blood using the BioRobot 9604 workstation

  • Rapid isolation of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Removal of contaminants and inhibitors

The QIAamp DNA Blood BioRobot 9604 Kit provides automated DNA purification on the BioRobot 9604 workstation using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot 9604 workstation requires less than 2.5 hours, including bar code reading and complete process documentation, with no hands-on time during the run.

Артикул: 965162
QIAamp DNA Blood BioRobot 9604 Kit (12)
For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Protease, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps


Performance

QIAamp 96-well plates provide well-to-well uniformity in DNA recovery and purity, and PCR experiments show no cross-contamination between samples in adjacent wells. As many as 96 samples of human whole blood or other body fluids can be processed yielding purified DNA in under 2 hours with minimal hands-on time. QIAamp sample preparation technology is fully licensed.

The QIAamp 96 DNA Blood procedure on the BioRobot 9604 increases productivity and reliability. DNA purified using the QIAamp 96 DNA Blood procedure on the BioRobot 9604 is free of contaminants and enzyme inhibitors, and is ready for use in amplification, Southern blotting, or other enzymatic assays for

  • Genetic disease testing
  • Identity testing
  • Cancer screening

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in three efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp technology yields genomic and mitochondrial DNA from blood and other body fluids ready to use in PCR and blotting procedures.

Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed with additional equipment and the use of a special protocol available from QIAGEN Technical Services or your local distributor.

Procedure

The QIAamp DNA Blood BioRobot 9604 Kit simplifies isolation of DNA from blood and other body fluids with a fast automated 96-well–plate procedure. Using the BioRobot 9604 Workstation, the QIAamp DNA Blood BioRobot 9604 Kit processes up to 200 µl sample size, with preparation time of 96 samples in less than 2 hours. The automated procedure, including bar code reading and process documentation, needs about 5 minutes hands-on time, with a turnaround time between consecutive runs of about 10 minutes.  The typical yield is 3–6 µg per 200 µl healthy whole blood, with an elution volume of 200 µl.

Applications

The QIAamp DNA Blood BioRobot 9604 Kit provides automated high-throughput DNA purification on the BioRobot 9604 using proven QIAamp technology. The kit processes 96-well plates for up to 96 preps. Sample types include:

  • Fresh or frozen whole blood
  • Serum
  • Bone marrow
  • Body fluids
  • Cell suspensions

Features
Specifications
Applications PCR, blotting
Elution volume 200 µl
For automated processing BioRobot 9604
Format 96-well plate
Main sample type Whole blood
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 200 µl
Technology Silica technology
Time per run or per prep
Yield 3–6 µg