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QIAcube HT

Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format

  • Reliable DNA, RNA and miRNA extraction from virtually any sample type
  • Mid- to high-throughput solution with dedicated purification kits saves costs and time
  • User-friendly software allows for easy data management and documentation
  • Innovative design features increase safety and minimize cross-contamination
  • Convenient, flexible and easy-to-use instrument with small footprint

QIAcube HT enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology. A variety of QIAcube HT Purification Kits lets users quickly and easily purify DNA, RNA and miRNA from almost any type of sample – including cells, tissues and stool samples, as well as from bacteria and viruses in animal samples. Automated protocols and dedicated accessories and consumables increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.

Артикул: 9022649
Accessories Pack, QXT
For upgrading QIAxtractor instruments to process dedicated QIAcube HT kits: Transfer Carriage, Riser Block EMTR, and Channeling Adapter
Артикул: 9001900
QIAcube HT Priority Package Plus
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package Plus with installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Артикул: 9001899
QIAcube HT Priority Package
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack; includes Priority Package with installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits
Артикул: 9001793
QIAcube HT System
Robotic workstation with UV lamp, HEPA filter, laptop, QIAcube HT operating software, startup pack, installation and training, 1-year warranty on parts and labor


QIAcube HT provides fast and reliable automated high-throughput nucleic acid purification for use in a variety of sensitive downstream applications. Researchers can easily increase their sample purification throughput and expect the same high-quality results as from other trusted QIAGEN nucleic acid purification solutions.

DNA from blood, cells and tissues

Automated purification of total DNA from blood, cells and tissue samples is quick, easy and reliable with QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed. QIAcube HT lets users increase sample purification throughput without having to compromise on quality or reliability, providing high yields of pure DNA that perform well in downstream analyses.

DNA from stool samples

Automated purification of DNA from stool samples is quick, easy and reliable with QIAcube HT and the dedicated QIAamp 96 PowerFecal QIAcube HT Kit. Whether stool samples from 10 different animals were subjected to manual gDNA purification using the QIAamp Fast DNA Stool Mini Kit or automated using the dedicated instrument kit, the target could be successfully amplified in a real-time PCR assay, as indicated by comparable CT values.

Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples. The QIAamp 96 PowerFecal QIAcube HT Kit efficiently eliminates PCR inhibitors from samples, enabling reliable detection of DNA, similar to other QIAGEN stool DNA purification solutions whether used manually or automated.

Viral DNA and RNA from a variety of samples

The QIAamp 96 Virus QIAcube HT Kit enables automated purification of viral RNA and DNA from a broad range of sample types including fresh or frozen tissues, blood and other body fluids. The procedure yields high-quality viral nucleic acids that perform well in downstream PCR and RT-PCR analyses. The procedure also provides high yields of pure RNA or DNA that perform well in downstream analyses, similar to other QIAGEN DNA purification solutions.

RNA from cells and tissues

RNA purification on QIAcube HT is simple and reliable with the RNeasy 96 QIAcube HT Kit and provides linear sample recovery with no significant well-to-well variation across a 96-well plate. This economical, easy-to-use system provides the same results as other trusted QIAGEN purification technologies.

Viral DNA and RNA and bacterial DNA from animal samples

QIAcube HT enables reliable purification of nucleic acids from undiluted animal whole blood with minimal risk of silica membrane clogging. Automated processing of samples in 96-well format is precise and repeatable between different purification runs performed on different days, providing not only convenience but also peace of mind. Co-purification of viral RNA and bacterial DNA is easily automated with QIAcube HT and dedicated cador Pathogen QIAcube HT Kit, and provides the same high-quality nucleic acids as manual processing with the QIAamp cador Pathogen Mini Kit. 

DNA from complex food samples

The DNeasy mericon 96 QIAcube HT Kit has been specially adapted for rapid mid- to high-throughput purification of DNA from a variety of raw and processed food matrices on the QIAcube HT instrument. A modified cetyltrimethylammonium bromide (CTAB) extraction procedure allows DNA extraction from all food samples in radically shorter time compared with conventional CTAB protocols. Now users can prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure.

The DNeasy mericon Kit can only be used with the QIAcube HT Prep Manager Software when requesting a customized protocol. The protocol is not included in the standard configuration of the Prep Manager software. However, all DNeasy mericon Kit protocols are pre-installed and compatible with the QIAcube HT Operating Software 4.17.


Automated purification on the QIAcube HT is quick and cost-effective and delivers high-quality nucleic acids from 24–96 samples per run. Dedicated QIAcube HT Kits enable purification of nucleic acids from a diverse range of starting materials, including cells, tissues, bacteria, viruses, stool and food material. The QIAcube HT is extremely easy to use, and the intuitive software lets users start a run with just a few mouse clicks.

Dedicated QIAcube HT Kits for a wide range of starting materials

Specimen Input Kit Eluate Elution volume
Fresh, frozen or treated blood, cells and tissues Blood: up to 200 µl
Cells: up to 5 x 106 cells
Tissues: up to 20 mg
QIAamp 96 DNA QIAcube HT Kit (5) Total DNA 200 µl (default)
Fresh or frozen stool Up to 200 mg QIAamp 96 PowerFecal Kit (5) Host and bacterial DNA 100 µl (default)
Fresh or frozen tissues, swabs, blood and any other body fluids Body fluids: up to 200 µl
Tissues: up to 20 mg
QIAamp 96 Virus QIAcube HT Kit (5) Viral DNA and RNA 100 µl (default)
Animal or human cells and tissues Cells: up to 5 x 106 cells
Tissues: up to 40 mg
RNeasy 96 QIAcube HT Kit (5) Total RNA including miRNA 110 µl (default)
Animal samples including whole blood, serum, plasma, body fluids, swabs, washes, tissues, feces Body fluids: up to 200 µl
Tissues: up to 25 mg
cador Pathogen 96 QIAcube HT Kit (5) Viral RNA
Viral/bacterial DNA
150 µl (default)
Raw or processed food matrices Up to 300 mg food material DNeasy mericon 96 QIAcube HT Kit (5) DNA 200 µl (default)

QIAcube HT is a compact, benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. QIAcube HT comes with a laptop computer for added flexibility.

Advanced instrument safety features, including a translucent hood, help to protect precious samples from environmental contamination. The HEPA filter maintains positive clean air pressure on the worktable underneath the hood and protects precious samples from potential airborne contamination. The UV light provides efficient worktable decontamination and helps to prevent cross-contamination. QIAcube HT ejects used tips through the tip disposal chute into a disposable plastic box located at the side of the instrument. This external tip ejection prevents waste accumulation in the clean workspace. The tip disposal box holder keeps the box positioned correctly under the tip chute. The disposal box also comes with a secure-fitting lid to make waste disposal simple and safe.


QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.

All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.

Liquid-handling tasks are performed by the 8-channel pipetting head, which provides fast transfer of liquids during the run. The software tracks tip use and internally updates tip availability as the run proceeds. QIAcube HT optimizes the use of plasticware with a smart and economical tip reuse strategy. After washing, the pipetting head moves the 96-well filter plate to the elution compartment, where purified nucleic acids are eluted into the elution microtubes. The included 8-well strip caps securely close the elution microtubes for sample storage.


QIAcube HT Software provides a user-friendly interface for operating the QIAcube HT instrument. The software wizard simulates the instrument worktable and makes it easy to set up and start a run with just a few mouse clicks. The software automatically calculates reagent volumes according to the number of samples, and fully documents every run. The software also allows you to import bar code databases, manage user access, and integrate Laboratory Information Management Systems (LIMS).

Accessories (supplied) Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly
Communications RS232 and USB
Consumables (supplied) QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml
Dimensions Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.)
Electrical 100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages
HEPA filter Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood.
Humidity Operating humidity 40-70%
Notebook (supplied) QIAcube HT comes with a notebook computer for added flexibility.
Pipetting system 8 pipetting channels, pipetting volume (20-200 µl)
Place of operation For indoor use only
Pollution level 2
Precision 20-200 µl, less than 4% coefficient of variation
Software Supplied with unlimited user license with free upgrades by web download
Table capacity 4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution
Throughput 24-96 samples in increments of 8 samples
Tip disposal All used tips ejected externally; no waste accumulation on the worktable
UV lamp Preinstalled UV lamp for worktable decontamination
Warranty 1-3 years warranty, depending on service package
Weight QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.)