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For rapid, high-precision automated PCR setup

  • Automated PCR setup in all formats
  • Convenient, easy-to-use software
  • Pre-optimized experiment setup
  • Standardized results and increased productivity
  • Elimination of manual pipetting steps

The QIAgility is a compact benchtop instrument that enables rapid, high-precision setup of PCR experiments with ready-to-use software for ease and convenience. The software provides step-by-step guidance for worktable setup and automatic calculation of all mixes, eliminating the need to program pipetting steps. Just select the kit and cycler, define your targets and start the run. For added flexibility the QIAgility is operated via a laptop computer.

QIAgility operates in conjunction with QIAgility Accessories and QIAgility Consumables.

Артикул: 9001904
QIAgility Priority Package Plus HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 3-year warranty on parts and labor, and 3 preventive maintenance visits
Артикул: 9001903
QIAgility Priority Package HEPA/UV (incl. PC)
Robotic workstation for automated PCR setup (with UV light and HEPA filter), notebook computer, and QIAgility Setup Manager Software: includes Priority Package with software, installation, training, 2-year warranty on parts and labor, and 2 preventive maintenance visits

Exceptional pipetting precision and accuracy spanning over a broad volume range

The Artel MVS system was employed to determine the pipetting precision of the QIAgility instrument operated by the QIAgility Setup Manager Software. The QIAgility dispensed the Artel solutions (selected according to the Artel MVS User Manual) into the Artel buffer. Three runs were performed on three QIAgility instruments that were used to pipet an entire volume range (1, 2, 5, 10, 20, 50, 100, and 200 µl), in replicates of 12. For each run and volume (n=12) the coefficient of variation (CV) was calculated to determine the pipetting precision of the QIAgility. The data shows the mean of the CV values (bars; in %) ± standard deviation (S.D.) of the CV values as error bars. The red lines depict the claimed specifications.

Unrivaled pipetting precision and reproducibility of the QIAgility for master mix solutions

A reaction mix was prepared manually using 5 parts of 2x Rotor-Gene SYBR Green PCR Master Mix and 3 parts of RNase free water (ready-made reaction mix). An experiment was then defined in the QIAgility Setup Manager for the Rotor-Gene SYBR Green PCR Kit with a total reaction volume of 25 µl and a sample input volume of 5 µl, resulting in the transfer of 20 µl of the ready-made reaction mix. The transfers were made into pre-weighed 200 µl single tubes (n=36 per instrument). The tubes were weighed again after the transfer and the weight difference was considered to calculate the transferred volume. For each instrument, the pipetting precision was determined as CV (%). The data shows the mean of the CV values (bars; in %) ± S.D. (error bars) of each individual instrument. The pipetting precision or CV, respectively, was nearly 3 % for all instruments tested.

Comparison of manual and automated assay setup using series dilutions

A QuantiNova SYBR Green assay setup was performed using the QIAgility operated with its Setup Manager Software and this was compared to a manual setup done in parallel. A manually prepared 1:10 dilution series from a stock of human gDNA with a concentration of 60 ng/µl was used in a ?-actin assay. Each dilution was tested in quadruplicates (n=4). The manual and QIAgility-prepared assay points were amplified in the same Rotor-Gene Q run. The data is represented as a box plot of the CT value calculated using the Q-Rex Software for each template concentration and setup employed.

High system linearity of QIAgility

Human gDNA with a stock concentration of 1.5 µg/µl was diluted with the QIAgility six times in the ratio of 1:2 per dilution step using RNase free water as diluent. The dilution series was prepared in replicates of 3. The nucleic acid concentration of the dilutions was determined using the QIAxpert UV/VIS spectrophotometer (DNA QIAamp Application). The mean measured quantifications were plotted against the mean theoretical nucleic acid concentrations assuming a perfect 1:2 dilution. The resulting plot demonstrates the exceptional linearity of QIAgility dilution with a high regression coefficient of >0.999.


The QIAgility fully automates proven QIAGEN PCR kits for use with various workflows, and offers pure pipetting precision, enhanced safety features, and exceptional reliability and reproducibility.

Unmatched pipetting precision with QIAgility

The QIAgility instrument operated by the QIAgility Setup Manager Software demonstrates exceedingly high accuracy and precision of dispensed volumes as verified by the Artel MVS system. These results indicate that the QIAgility can produce automated, high-performance liquid dispensing results, while avoiding common errors that can be associated with manual pipetting and thereby reducing the time to result.
Regardless of whether you pipet water or QIAGEN ready-made reaction mix to set up a PCR experiment, the QIAgility instrument can dispense volumes that are highly precise and also generate significantly reproducible replicates.

High performance PCR setup using QIAgility

A QuantiNova SYBR Green PCR assay setup was performed manually and automated in parallel using the QIAgility along with its Setup Manager Software. The amplification run was done on the Rotor-Gene Q and resulting data analyzed using the Q-Rex Software. Consistent results were obtained from both manual and automated reaction setup using the same template dilutions.

Exceptional linearity of QIAgility

QIAgility exhibits excellent linearity and assures accurate and reliable use of sample for an assay. To assess the linearity of the system, pure gDNA was serially diluted using the QIAgility and measured for their concentrations on the QIAxpert. The quantifications from the QIAxpert were plotted against the known concentration values, and the resulting plot demonstrates the exceptional linearity.


Automated PCR setup is rapid and reliable and eliminates manual pipetting steps that are typically prone to human error.

Liquid-handling tasks are performed by the single-channel pipetting head, which provides fast transfer of liquids with various types of plasticware. For example, 96 samples with the QuantiFast SYBR Green PCR Kit with 2 standard series can be setup in approximately 45 minutes. The worktable features a flexible layout and supports almost all kinds of cyclers and labware, giving high application versatility. Dynamic liquid-level sensing, facilitated by conductive filter tips offers maximum safety control.

The advanced design of the QIAgility delivers improved process safety, giving you peace of mind. A built-in sensor ensures that the instrument pauses when the hood is opened during a run, protecting users from moving parts. The UV light ensures effective worktable decontamination and the HEPA filter provides positive clean air pressure throughout PCR setup.

QIAgility is a compact benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. For added flexibility, the QIAgility comes with a laptop computer. To reduce your work load, comprehensive run reports containing all run information are generated automatically during each protocol.


Quick and easy setup of your PCR experiment with the new Setup Manager Software.


The instrument is highly suited for laboratories performing PCR and RT-PCR reactions with applications in:

  • Gene expression analysis
  • Genotyping
  • Cloning
  • Pathogen detection
  • Food and veterinary testing


Is your plate or tube not supported in the QIAgility software? Contact QIAGEN Technical Services to request a new plate or tube to suit your workflow.

Accessories (supplied) 2 x Adapter, 96 x 0.2 ml PCR Tubes 1 x Adapter, Flip-Cap 32 x 1.5 ml Tapered Base 1 x Adapter, 72 x 0.1 ml Strip Tubes 1 x Master Mix Block, 5 x 5 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 2 ml 1 x Master Mix Block, 1 x 5 ml, 4 x 1.5 ml 1 x Reagent Block, 16 x 0.2 ml / 2 ml Flat / 1.5 ml Tapered Tubes 4 x Tip Rack Holder for 96-Tip Rack The adapters are intended to hold tubes or plates.
Communications USB and RS232
Conductive Filtered Tips 50 µl and 200 µl Conductive Filtered Tips for liquid-level sensing
Consumables (supplied) 10 racks of 50 µl Conductive Filtered Tips (960); 10 racks of 200 µl Conductive Filtered Tips (960); 25 x 5 ml tubes; 100 x 0.2 µl PCR tubes; 3 Tip Disposal Boxes
Dimensions Height: 45 cm (18 in.), instrument lid closed; Height: 86 cm (34 in.), instrument lid open; Width: 54 cm (21 in.); Depth: 63 cm (25 in.), without cables
Electrical 100–240 V AC, 250 VA, 50–60 Hz
HEPA filter Preinstalled dual-cartridge 3M Air-Mate HEPA (High Efficiency Particulate Air) filter system provides positive pressure clean air within the closed hood.
Humidity Operating humidity: 40–70%
Liquid-level sensing Minimum detection volume (10 µl in 200 µl PCR tubes). Only functional in combination with QIAGEN Conductive Filtered Tips.
Notebook (supplied) QIAgility comes with a notebook computer for added flexibility.
Pipetting head Single channel
Place of operation For indoor use only
Pollution level 2
Precision CV
Software Supplied with unlimited user license. Free upgrades by Web download.
Table capacity 6 x SBS positions (127.76 x 85.48 mm) for adapters and Tip Rack Holders 1 x Master Mix Block 1 x Reagent Block
Temperature Operating temperature, 15–30 °C (59–86°F)
Tip disposal All used tips ejected externally — no waste accumulation on the worktable
Tip volume 1–200 µl
UV lamp Preinstalled UV lamp for worktable decontamination
Warranty 1-3 years warranty, depending on service package
Weight 41 kg (90.2 lb)