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QIAquick Nucleotide Removal Kit

For up to 10 μg oligonucleotide (17–40mers) and DNA (40 bp to 10 kb) cleanup from enzymatic reactions

  • Up to 95% recovery of ready-to-use DNA
  • Fast and convenient procedure
  • Cleanup of DNA up to 10 kb in three easy steps
  • Gel loading dye for convenient sample analysis

The QIAquick Nucleotide Removal Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of oligonucleotides and DNA. Unincorporated nucleotides, salts, and other contaminants are removed and oligonucleotides (>17 nt) and DNA fragments ranging from 40 bp to 10 kb are purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–200 ?l. The procedure is fully automated on the QIAcube.

Артикул: 28306
QIAquick Nucleotide Removal Kit (250)
250 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
Артикул: 28304
QIAquick Nucleotide Removal Kit (50)
50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
Артикул: 28115
QIAquick Spin Columns (100)
For DNA fragment purification from PCR, gel fragments and enzymatic reactions

Performance

The QIAquick Nucleotide Removal procedure removes nucleotides, enzymes, salts, and other impurities from DNA samples. Using a microcentrifuge, 17mer – 10 kb DNA is purified. For sample volumes smaller than 50 µl the DyeEx Spin kit can also be used.

Principle

QIAquick Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, agarose, ethidium bromide, and other impurities from DNA samples. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments from migrating too far.

Procedure

The QIAquick system uses a simple bind–wash–elute procedure. Binding buffer is added directly to the sample, and the mixture is applied to the QIAquick spin column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.

Handling

QIAquick spin columns are designed to provide two convenient handling options. The spin columns fit into a conventional table-top microcentrifuge. The QIAquick Nucleotide Removal Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results.

Applications

DNA fragments purified with the QIAquick system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, and microinjection.

Features
Specifications
Binding capacity 10 µg
Elution volume 30–50 µl
Format Tube
Fragment size 40 bp – 10 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: oligonucleotides, dsDNA
Removal Removal
Sample type: applications DNA, oligonucleotides: PCR reactions
Technology Silica technology