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DNeasy Plant Mini Kit

For isolation of up to 30 µg total cellular DNA from plant cells and tissues, or fungi

  • Pure DNA, free from contaminants and enzyme inhibitors
  • Rapid isolation of ready-to-use DNA
  • No organic extraction, no ethanol precipitation

The DNeasy Plant Mini Kit provides fast and easy silica-based DNA purification in convenient spin column format. Typical yields are 3–30 ?g of high-quality DNA, depending on the samples used. Purification of DNA using the DNeasy Plant Mini Kit can be automated on the QIAcube Connect.

DNeasy Plant Pro Kit – superior DNA yields and inhibitor removal from plant samples. See new kit

Артикул: 69106
DNeasy Plant Mini Kit (250)
250 DNeasy Mini Spin Columns, 250 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)
Артикул: 69104
DNeasy Plant Mini Kit (50)
50 DNeasy Mini Spin Columns, 50 QIAshredder Mini Spin Columns, RNase A, Buffers, Collection Tubes (2 ml)


The DNeasy Plant Mini Kit allows rapid and efficient isolation of high-quality DNA from a wide variety of plant species and tissue types including the most demanding sources. Samples may be fresh, frozen, or dried. The optimized DNeasy Plant procedure incorporates the QIAshredder spin column, a unique filtration and homogenization column that efficiently removes cell debris and improves sample handling following lysis.

Selection of plant species processed with DNeasy Kits

Abies alba (silver fir) Nicotiana tabacum (tobacco)
Aesculus hippocastanum (horse chestnut) Oryza sativa (rice)4
Arabidopsis thaliana (thale cress) Pelargonium sp. (geranium)4
Avena sp. (oat) Petunia sp.4
Brassica napus (oilseed rape) Pinus sylvestris (Scotch pine), P. brutia5
Brassica oleracea (kohlrabi) Populus tremula (aspen)
Chicorium endivia (chicory) Pseudotsuga menziesii (Douglas fir)
Citrullini lanatus (water melon) Quercus robur, Q. petrea (oak)6,7
Egeria sp. Rhododendron sp.2,4
Fagus sylvatica (beech)1 Rubus idaeus (raspberry)
Helianthus spp. (sunflower) Solanum tuberosum (potato)
Hordeum vulgare (barley)2 Sphagnum palustre (moss)
Humulus sp. (hops) Spinacia oleracea (spinach)
Hydrilla sp. Taxus baccata (yew)
Kalanchoe spp. Triticum aestivum (wheat)4
Lupinus sp. Ulmus glabra (elm)6
Lycopersicon esculentum (tomato)3 Vitis spp. (grape)6
Myriophyllum sp. Zea mays (maize)
Young leaves or needles (and other tissues, as indicated) were collected and immediately flash frozen. DNA isolation was then performed with the DNeasy Plant Mini Kit. 1Beechnut, 2dried leaves, 3callus, 4leaves from adult plant, 5endosperm, 6old leaves, rich in carbohydrates, 7buds. For more information on DNA isolation from other species including fungi, call QIAGEN Technical Services or your local distributor.

The typical yield is 3–30 µg, with a sample size of up to 100 mg wet weight, and an elution volume of 50–400 µl. The DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and age of tissue sample. Lower and higher range values correspond to arabidopsis and wheat, respectively.

The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. Absorbance scans of DNeasy purified DNA show a symmetrical peak at 260 nm, confirming that the DNA is free of impurities, including enzyme inhibitors. DNeasy purified DNA is sized up to 40 kb. Purified DNA can be used in a wide range of applications.


DNeasy Plant Kits use advanced silica-membrane technology and simple spin procedures to isolate highly pure total cellular DNA from plant tissues and cells or fungi. DNeasy technology replaces cumbersome DNA isolation procedures such as cetyltrimethylammonium bromide (CTAB), phenol, or chloroform extraction. Using the DNeasy procedure, alcohol precipitation is not necessary — purified DNA is ready for immediate use. DNeasy sample preparation technology is fully licensed.


Samples are first mechanically disrupted and then chemically lysed. RNA is removed by RNAse digestion during lysis. Cell debries, precipitated proteins, and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted and the lysate is loaded onto the DNEasy Plant Mini spin column. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use.


The DNeasy Plant Mini Kit provides purification of DNA from plant tissue, including:

  • Plant cells
  • Plant tissues
  • Fungi

Applications PCR, real-time PCR, blotting
Elution volume 50–400 µl
Format Spin column
Main sample type Plant samples
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount
Technology Silica technology
Time per run or per prep
Yield 3–30 µg