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Каталог

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FlexiGene DNA Kit

For scalable isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube

  • Easy handling, no risk of sample mix-up
  • Scalable purification from 0.1–20 ml whole blood
  • Direct purification from whole blood, buffy coat, and cultured cells
  • Fast procedure
  • No organic extraction

The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.

Артикул: 51206
FlexiGene DNA Kit (250)
For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation


Performance

DNA purified using the FlexiGene system is sized up to 150 kb. It performs well in amplification and Southern blotting experiments, and is also suitable for archiving.

DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 106 cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.

DNA yields purified from different sample types using the FlexiGene procedure

Sample Volume
Blood*  0.3 ml 1–14
Blood*  2.0 ml 75–90
Blood*   10.0 ml  380–460
Buffy coat   0.5 ml   110–130
HeLa cells   106 cells   25–35
* Whole blood with normal white blood cell counts (7x106 cells/ml blood).

Principle

The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.

Procedure

The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20?C. High DNA yields are obtained from whole blood, buffy coat, and cultured cells (see table "DNA yields purified from different sample types using the FlexiGene procedure").

Applications

DNA purified using the FlexiGene DNA kit performs well downstream applications, including,

  • PCR
  • Southern blotting
  • Archiving

Features
Specifications
Applications PCR techniques, restriction digest, Southern blotting, sequencing
Elution volume 200 µl - 1000 µl
Format Scalable
Main sample type Whole blood, buffy coat, cultured cells
Processing Manual
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 100 µl - 20 ml
Technology Single tube precipitation method
Time per run or per prep 25 - 45 minutes (+ DNA rehydration)
Yield 35 µg / ml whole blood