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Каталог

Каталог

MagAttract 96 DNA Plant Core Kit

For efficient, high-throughput, manual or automated purification of total cellular DNA from plant tissue

  • An economical solution
  • Reproducible yields of pure, ready-to-use DNA
  • A simple, rapid, and reliable procedure
  • A manual protocol for initial evaluation

The MagAttract 96 DNA Plant Core Kit combines the efficiency of silica-based DNA purification with the convenient handling offered by magnetic particles, in manual, semi-automated, or automated 96-well plate format.

Артикул: 67163
MagAttract 96 DNA Plant Core Kit (24)
MagAttract Suspension and Buffers for 24 x 96 preps
Артикул: 19090
Buffer RPW (concentrate, 125 ml)
Buffer RPW (concentrate, 125 ml)


Performance

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Purification using the MagAttract 96 DNA Plant Core Kit

Format: 96-well sample plates
Sample sources: Plant tissue
Plant starting material, fresh or frozen: 10–100 mg 
Plant starting material, dried or lyophilized: 10–30 mg
Typical yield: 1–15 µg
Elution volume: 100 µl

Purified DNA has been used successfully in PCR and real-time PCR.

Principle

The MagAttract 96 DNA Plant Kit is designed for fully automated, high-throughput preparation of genomic and chloroplast DNA from plant tissue. Proven MagAttract magnetic particle technology in combination with the BioRobot Plant Science system (no longer available) provides efficient and reproducible DNA purification.

Procedure

Plant samples are mechanically disrupted using a homogenizer such as the TissueLyser. The resulting powder is resuspended in lysis buffer, thoroughly mixed, and centrifuged. Lysates are transferred to a 96-well microplate containing MagAttract magnetic particles, and genomic DNA selectively binds to the surface of the particles. After washing, pure genomic DNA is eluted with low-salt buffer, and is ready for use in downstream applications. The MagAttract 96 DNA Plant Kit is designed for use with the BioRobot Plant Science system for fully automated DNA purification. QIAGEN also offers an automated protocol using the BioRobot RapidPlate workstation, and a manual protocol for evaluation purposes.

Applications

The MagAttract 96 DNA Plant Kit provides high yields of DNA from many plant species. The purified DNA is up to 40 kb in size, with fragments of 23 kb predominating, and is ready to use in downstream applications, including:

  • PCR
  • Real-time PCR
  • Microsatellite analysis
  • SNP genotyping