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Каталог

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QIAamp 96 PowerFecal QIAcube HT Kit

For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors

  • Simple and reliable automated processing for cost and time savings
  • No hazardous organic chemicals required
  • Efficient removal of PCR inhibitors
  • High sensitivity in downstream assays

The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO's patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.

Артикул: 950067
QIAcube HT Plasticware
Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
Артикул: 51531
QIAamp 96 PowerFecal QIAcube HT Kit (5)
For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers
Артикул: 19585
S-Blocks (24)
96-well blocks with 2.2 ml wells, 24 per case
Артикул: 19092
Pathogen Lysis Tubes L
50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX


Performance

QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.

Effective removal of inhibitors

Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.

Principle

The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.

Procedure

The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA.

Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.

Applications

The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.

Features
Specifications
Elution volume 100 ?l
Sample amount Up to 200 mg fresh or frozen stool
Throughput 24-96 samples in increments of 8 samples
Time per run 96 samples in approximately 120 minutes 24 samples in approximately 60 minutes