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Каталог

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QIAamp DNA Blood Mini Kit

For purification of up to 12 µg genomic, mitochondrial, or viral DNA from blood and related body fluids

  • Rapid purification of high-quality, ready-to-use DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors for reliable results

The QIAamp DNA Blood Mini Kit provides silica-membrane-based DNA purification. The QIAamp DNA Blood Mini Kit is designed for processing up to 200 ?l fresh or frozen human whole blood. QIAamp Mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Purification of DNA using the QIAamp DNA Blood Mini Kit can be fully automated on the QIAcube Connect.

Артикул: 51106
QIAamp DNA Blood Mini Kit (250)
For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
Артикул: 51104
QIAamp DNA Blood Mini Kit (50)
For 50 DNA minipreps: 50 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)

Performance

QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples. The purified DNA is suitable for long-range PCR amplification.

Principle

No phenol–chloroform extraction is required. DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acid to be eluted in either water or a buffer provided with the kit. QIAamp DNA Blood technology yields genomic, mitochondrial, or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures.

Procedure

The QIAamp DNA Blood Mini Kit simplifies isolation of DNA from blood and related body fluids with fast spin-column or vacuum procedures. Fresh and frozen whole blood with common anticoagulants, such as citrate, EDTA, and heparin, may be processed. The QIAamp DNA Blood Mini Kit processes sample sizes of up to 200 µl, with a preparation time of 20–40 minutes. The typical yield from 200 µl healthy whole blood is 4–12 µg, with an elution volume of 50–200 µl. The QIAamp DNA Blood Mini Kit procedure is automatable.

QIAamp sample preparation technology is fully licensed.

Vacuum processing

With the QIAamp DNA Blood Mini Kit, blood can be processed by vacuum instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp mini spin columns are accommodated on the QIAvac 24 manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows these QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters.

Applications

The QIAamp DNA Blood Mini Kit provides proven QIAamp technology for purification of DNA from a variety of materials using Mini spin columns with 2.0 ml collection tubes. Sample sources include:

  • Fresh and frozen whole blood or buffy coat
  • Plasma or serum
  • Bone marrow
  • Lymphocytes
  • Platelets
  • Body fluids

Features
Specifications
Applications PCR, long-range PCR, Southern blotting
Elution volume 50–200 µl
Format Spin column
Main sample type Whole blood, body fluids
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA, viral DNA
Sample amount 1–200 µl
Technology Silica technology
Time per run or per prep 20–40 minutes
Yield 4–12 µg