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Каталог

Каталог

QIAamp DNA Micro Kit

For purification of genomic and mitochondrial DNA from small samples

  • Rapid purification of high-quality DNA
  • No organic extraction or alcohol precipitation
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors

The QIAamp DNA Micro Kit simplifies purification of genomic and mitochondrial DNA with a fast spin-column procedure. Optimized protocols are included for use with small amounts of fresh or frozen blood, tissue, and dried blood spots. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 ?l. Purification of DNA using the QIAamp DNA Micro Kit can be automated on the QIAcube.

Артикул: 56304
QIAamp DNA Micro Kit (50)
For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)


Performance

The resulting purified DNA is free of proteins, nucleases, and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR and laser microdissection (LMD) PCR. DNA purified with the QIAamp DNA Micro Kit may also be used in short-tandem repeat (STR) genotyping, single-nucleotide pylomorphism (SNP) genotyping, or pharmacogenomic research.

Principle

The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µl. DNA binds specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. No phenol–chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA Micro technology yields genomic and mitochondrial DNA from small samples ready to use in PCR and blotting procedures.

QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

Procedure

The QIAamp DNA Micro Kit simplifies isolation of DNA from small samples with a fast spin-column procedure. The QIAamp DNA Micro Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes (e.g., 1–100 µl whole blood, blood cards of ~3 mm diameter, urine, or <10 mg tissue or laser microdissected tissue). Fresh or frozen blood, tissue, and dried blood spots can be purified in 30 minutes and eluted in 20–100 µl.

Applications

The QIAamp DNA Micro Kit procedure is suitable for a wide range of sample materials, including:

  • Small volumes of blood
  • Blood cards
  • Urine
  • Small tissue samples, including laster microdissections

Features
Specifications
Applications Real-time PCR, STR analysis, LMD-PCR
Elution volume 20–100 µl
Format Spin column
Main sample type Whole blood
Processing Manual (centrifugation or vacuum)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA, mitochondrial DNA
Sample amount 1–100 µl
Technology Silica technology
Time per run or per prep 30 minutes
Yield