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Каталог

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QIAGEN Plasmid Plus 96 Kits

For purification of up to 50 µg transfection-grade plasmid DNA in 96-well format

  • Transfection-grade plasmid DNA with endotoxin levels <1 EU/µg DNA
  • DNA purity comparable to anion-exchange technology
  • High yields (up to 50 µg)
  • Convenient handling and simple procedure
  • Vacuum, centrifugation, and automated processing options

QIAGEN Plasmid Plus 96 Kits provide a novel method for plasmid preparation in 96-well format. Samples can be conveniently processed using the QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Proprietary binding chemistry allows up to 50 ?g of transfection-grade plasmid DNA per well to be obtained from up to 5 ml of an E. coli culture. Endotoxin levels are reduced to levels below 1 EU/µg DNA due to a dedicated endotoxin reduction buffer.

For optimal results it is recommended to use this product together with QIAvac 96.

Артикул: 960241
QIAGEN Plasmid Plus 96 BioRobot Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates and Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; for use with the BioRobot Universal System
Артикул: 16181
QIAGEN Plasmid Plus 96 Miniprep Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Services), or a centrifugation system suitable for 96-well blocks


Performance

Consistently high yields of ready-to-use plasmid DNA

QIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. High yields of up to 50 ?g of transfection-grade plasmid DNA can be obtained.

Very low endotoxin levels

Kits include an endotoxin reduction buffer that effectively reduces bacterial endotoxins to levels typically lower than 1 EU/?g DNA, providing plasmid DNA quality which is suitable for transfection into sensitive cell lines.

Principle

QIAGEN Plasmid Plus 96 Kits are based on QIAGEN Plasmid Plus technology, a novel method for plasmid preparation. The procedure is based on a nonchaotropic binding chemistry that ensures selectivity and DNA quality that is comparable to anion exchange preps. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications. The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities.

Procedure

This novel method for plasmid preparation in 96-well format provides highly pure, transfection-grade plasmid DNA using a simple bind-wash-elute procedure.

The QIAGEN Plasmid Plus 96 Miniprep protocol starts with a modified alkaline lysis procedure. After neutralization, lysates are cleared using a TurboFilter 96 plate. A nonchaotropic binding buffer is added to the cleared lysate to optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate. The unique binding buffer provides very specific binding conditions leading to DNA quality that is comparable to anion-exchange preps. A wash step with an endotoxin reduction buffer results in transfection-grade plasmid DNA suited for transfection into most cell lines.

Convenient and flexible processing options

The procedure may be fully automated using the QIAGEN Plasmid Plus 96 BioRobot Kit on the BioRobot Universal System. Alternatively, the QIAvac 96 is used for lysate clearing using the TurboFilter 96 plate, and DNA binding and wash steps are performed using the Plasmid Plus 96 plate. Drying and elution steps may either be performed on the QIAvac 96 or using a compatible centrifuge. In addition, the complete procedure can be performed using a compatible centrifuge.

Applications

Plasmid DNA purified using QIAGEN Plasmid Plus 96 Kits is highly suitable for a wide variety of applications, including:

  • Transfection, including into sensitive cell lines
  • Preparation of short hairpin vectors (sh-vectors)
  • Enzymatic modifications
  • Cloning
  • Restriction digestion

Features
Specifications
Applications Transfection into sensitive cell lines, preparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing, etc
Culture volume/starting material 1.25–5 ml culture volume
Plasmid type High-copy, low-copy, cosmid DNA
Processing QIAvac 96, centrifuge, automated
Samples per run (throughput) 48–96 samples per run
Technology QIAGEN Plasmid Plus technology
Time per run or prep per run 50 minutes
Yield 15–50 µg