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Каталог

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QIAGEN Plasmid Plus Kits

For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA

The QIAGEN Plasmid Plus Kits enable ultrafast, large-scale purification of up to

10 mg of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing hands-on time. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The QIAGEN Plasmid Plus Kits also feature a dedicated wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.

For optimal results it is recommended to use this product together with QIAvac 24 Plus.

Артикул: 19418
QIAvac Holder
Holder for stabilizing QIAGEN Plasmid Plus Mega and Giga columns with Tube Extenders on the QIAvac 24 Plus
Артикул: 19408
VacValves (24)
24 valves for use with the QIAvac 24 and QIAvac 24 Plus
Артикул: 12991
QIAGEN Plasmid Plus Giga Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Артикул: 12981
QIAGEN Plasmid Plus Mega Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Артикул: 12965
QIAGEN Plasmid Plus Maxi Kit (100)
100 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Maxi Cartridges
Артикул: 12963
QIAGEN Plasmid Plus Maxi Kit (25)
25 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Maxi Cartridges
Артикул: 12945
QIAGEN Plasmid Plus Midi Kit (100)
100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
Артикул: 12943
QIAGEN Plasmid Plus Midi Kit (25)
25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges
Артикул: 12941
QIAGEN Plasmid Plus Midi Kit (5)
QIAGEN Plasmid Plus Midi Kit (5)

Performance

Consistent quality and performance

QIAGEN Plasmid Plus technology delivers the same performance and quality as anion-exchange technology. The unique binding chemistry of the QIAGEN Plasmid Plus columns ensures that pure plasmid DNA is obtained every time. High-yield protocols and extra buffer volumes are provided with the QIAGEN Plasmid Plus Kits. Yields of up to 10 mg (giga), 2 mg (mega), 1 mg (maxi), and 250 ?g (midi) of highly pure plasmid DNA, free of contaminants such as RNA, proteins, and genomic DNA are achieved.

Transfection-grade plasmid DNA with very low endotoxin levels

Endotoxins, also known as lipopolysaccharides or LPS, are cell-membrane components of Gram-negative bacteria such as E. coli and are released during the lysis step of plasmid purification. Endotoxins can induce nonspecific activation of immune responses, and thus represent a noncontrollable variable in transfection experiment setup, influencing the outcome and reproducibility of results and making them difficult to compare and interpret.  

The endotoxin wash buffer provided in the kits reduces bacterial endotoxins to levels usually below 1 EU/?g DNA. (Endotoxin levels can vary depending on the host strain, culture media, and cell density. If the cell density is very high, endotoxin levels may be slightly higher, ranging from 13 EU/?g DNA.) This makes the plasmid DNA suitable for a multitude of applications, including transfection into sensitive cell lines. 

Principle

The QIAGEN Plasmid Plus Kits provide the most convenient method for large-scale plasmid preparation. The design and unique binding chemistry of the QIAGEN Plasmid Plus columns allow a simple bind-wash-elute procedure based on a novel chemistry. This procedure makes these kits the fastest large-scale prep available 20 minutes for maxi and midi preps. The resulting plasmid DNA is highly concentrated and ready for immediate use in subsequent applications without further ethanol precipitation. Thus, the plasmid DNA is highly suitable for applications that only allow small reaction volumes.

Maximum plasmid yields using LyseBlue

LyseBlue, an optional color indicator that provides visual identification of optimum buffer mixing, is also included in the kit. This prevents common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, genomic DNA, and cell debris. Through a simple color change, this unique buffer additive ensures that optimum cell lysis and neutralization is achieved, maximizing yields.

Specifications

Features Plasmid Plus
Giga Kit
Plasmid Plus 
Mega Kit
Plasmid Plus 
Maxi Kit
Plasmid Plus 
Midi Kit
Applications Transfection of most cell lines, cloning, enzymatic restriction,
in-vitro translation/transcription, sequencing
Transfection of most cell lines, cloning, enzymatic restriction,
in-vitro translation/transcription, sequencing
Transfection of most cell lines, cloning, enzymatic restriction,
in-vitro translation/transcription, sequencing
Transfection of most cell lines, cloning, enzymatic restriction,
in-vitro translation/transcription, sequencing
Culture volume/starting material 2500 ml culture volume 500 ml culture volume 50–200 ml culture volume 25–50 ml culture volume
Final DNA concentration ?1 µg/µl ?1 µg/µl ?1 µg/µl ?1 µg/µl
Plasmid type Plasmids, cosmids Plasmids, cosmids Plasmids, cosmids Plasmids, cosmids
Processing Manual (centrifugation and vacuum), parallel processing Manual (centrifugation and vacuum), parallel processing Manual (centrifugation and vacuum), parallel processing Manual (centrifugation and vacuum), parallel processing
Samples per run; throughput 1–12 samples per run 1–12 samples per run 1–24 samples per run 1–24 samples per run
Technology Advanced silica technology (non-chaotrophic), endotoxin reduction Advanced silica technology (non-chaotrophic), endotoxin reduction Advanced silica technology (non-chaotrophic), endotoxin reduction Advanced silica technology (non-chaotrophic), endotoxin reduction
Time per run 50 min 40 min  20 min 20 min
Yield up to 10 mg up to 2.5 mg up to 1 mg up to 250 µg

Procedure

QIAfilter cartridges provided in QIAGEN Plasmid Plus Kits enable rapid lysate clearing. Loading of the cleared lysate onto the column can be performed in one step, saving time. A vacuum manifold (such as the QIAvac 24 Plus) is used to draw the cleared lysate and subsequent wash buffers through the QIAGEN Plasmid Plus column. Following the washing and drying of the membrane, the highly concentrated DNA is eluted in low volumes by centrifugation.

Applications

The QIAGEN Plasmid Plus Kits provide transfection-grade plasmid DNA highly suited for all applications, such as:

  • Transfection into most cell lines (e.g., sensitive cell lines such as Huh-7)
  • Restriction digestion
  • Cloning
  • In vitro transcription and translation
  • Sequencing

Features
Specifications
Applications Transfection of most cell lines, cloning, enzymatic restriction, in-vitro translation/transcription, sequencing
Culture volume/starting material 50–200 ml culture volume
Final DNA concentration ? 1 µg/µl
Plasmid type Plasmids, cosmids
Processing manual (centrifugation and vacuum), parallel processing
Samples per run (throughput) 1–24 samples per run
Technology Advanced Silica technology (non-chaotrophic), Endotoxin reduction
Time per run or prep per run 20 minutes
Yield up to 1mg