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QIAprep 96 Plus Kits

For purification of up to 50 μg high-quality plasmid DNA in 96-well format

QIAprep 96 Plus Kits enable plasmid preparation in 96-well format. Samples can be processed using QIAvac 96 and/or a centrifuge or automated on the BioRobot Universal System. Up to 50 ?g of high-quality plasmid DNA (endotoxin levels <10 EU/?g DNA) can be obtained from up to 5 ml of culture.

For optimal results it is recommended to use this product together with QIAvac 96.

Артикул: 962241
QIAprep 96 Plus BioRobot Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; for use with the BioRobot Universal System
Артикул: 27291
QIAprep 96 Plus Miniprep Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.


Performance

High-purity preps and outstanding yields

QIAprep 96 Plus Kits provide a novel method for preparation of highly pure DNA. Yields of up to 50 ?g can be consistently achieved using an optimized procedure. The unique binding buffer provided with the kits ensures very specific binding conditions.

Principle

The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps. Due to unique kit chemistry, up to 50 ?g plasmid DNA per well can be obtained from a 5 ml E. coli culture .

Procedure

QIAprep 96 Plus Kits allow plasmid preparation in 96-well format. The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications.

Convenient and flexible processing

The QIAprep 96 Plus Miniprep Kit can be conveniently processed using the QIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal System using the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforward plasmid DNA preparation in 96-well format.

Applications

Plasmid DNA purified using QIAprep 96 Plus Kits is highly suitable for a wide range of applications, including:

  • Preparation of short hairpin vectors (sh-vectors)
  • Enzymatic modifications
  • Cloning
  • Restriction digestion
  • Sequencing

Features
Specifications
Applications Preparation of short hairpin vectors (sh-vectors), enzymatic modifications, cloning, restriction digestion, sequencing, etc
Culture volume/starting material 1.25–5 ml culture volume
Plasmid type High-copy, low-copy, cosmid DNA
Processing QIAvac 96, centrifuge, automated
Samples per run (throughput) 48–96 samples per run
Technology Nonchaotropic binding chemistry
Time per run or prep per run 40 minutes
Yield 15–50 µg