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QIAsymphony Virus/Bacteria Kits

For automated purification of viral nucleic acids or bacterial DNA from 1–96 samples

  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents
  • Optimized protocols provide high nucleic acid yields
  • Novel, prefilled reagent cartridges for ease of use

QIAsymphony Virus/Bacteria Kits provide automated purification of viral nucleic acids and bacterial DNA from a broad range of sample types on the QIAsymphony SP. For example, nucleic acids can be purified from plasma, serum, CSF, respiratory samples, urine, urogenital swabs, and transport media. Kits are available in mini and midi format for sample volumes of 200 ?l and up to 1000 ?l. Optimized protocols provide high yields of pure nucleic acids, and flexible elution volumes allow nucleic acid concentration to be easily optimized for each downstream application. Prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Артикул: 931055
QIAsymphony Virus/Bacteria Midi Kit (96)
For 96 preps of 1000 µl each: includes 2 reagent cartridges and enzyme racks and accessories
Артикул: 931036
QIAsymphony Virus/Bacteria Mini Kit (192)
For 192 preps of 200 µl each: includes 2 reagent cartridges and enzyme racks and accessories


Performance

QIAsymphony Virus/Bacteria Kits enable robust and reproducible capture of nucleic acids. Purified viral and bacterial nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications. Efficient yields and flexible elution volumes (60 ?l, 85 ?l, or 110 ?l) to suit the requirements of downstream applications ensure sensitive analytical detection.

Principle

QIAsymphony Virus/Bacteria Kits combine the speed and efficiency of silica-based purification of viral and bacterial nucleic acids with the convenient handling of magnetic particles. Innovative, ready-to-run reagent cartridges are prefilled with magnetic particles and all reagents required for the purification procedure, including accessory enzymes and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination. Worktable setup is rapid, which saves your valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute. The user can choose between different elution volumes depending on the protocol. The QIAsymphony SP performs all steps of the purification procedure. Up to 96 samples are processed in a single run.

QIAsymphony Virus/Bacteria protocols

Protocol Kit  Sample material  Processed volume
Cellfree 200   QIAsymphony Virus/Bacteria Mini Kit (192) Plasma, serum, and CSF   200 ?l
Cellfree 500    QIAsymphony Virus/Bacteria Midi Kit (96)  Plasma, serum, and CSF   500 ?l
Cellfree 1000    QIAsymphony Virus/Bacteria Midi Kit (96)  Plasma, serum, and CSF   1000 ?l
Complex 200   QIAsymphony Virus/Bacteria Mini Kit (192)   Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)    200 ?l
Complex 400   QIAsymphony Virus/Bacteria Midi Kit (96)   Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)   400 ?l 
Complex 800     QIAsymphony Virus/Bacteria Midi Kit (96) Respiratory samples (BAL, dried swabs, transport media, aspirates, sputum), and urogenital samples (urine, transport media)   800 ?l 

Applications

QIAsymphony Virus/Bacteria Kits enable highly sensitive detection of a broad range of DNA and RNA viruses, as well as bacterial pathogens. Yields are linear, allowing accurate quantitative analysis for both low and high viral or bacterial titers. The high-quality nucleic acids are ready to use in all downstream applications, including sensitive detection assays using quantitative, real-time PCR or RT-PCR.

Features
Specifications
Applications PCR, real-time PCR
Elution volume 60-110 µl
Input volume 800 µl (Pathogen Complex Protocol); 1000 µl (Virus Cellfree Protocol)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Purification of viral RNA and DNA and bacterial DNA
Sample types Serum, plasma, CSF, cell-free body fluids, fresh or frozen material (body fluids, swabs, secretions, tissues, stool specimens)
Technology Magnetic particles